000			01674nab a2200193 4500
005			20260520002031.0
008			260224s2011 xxu
100	1		_aXu, Yaohui _952309
100	1		_aLu, Mang _952310
245	0	0	_aMicrobially enhanced oil recovery at simulated reservoir conditions by use of engineered bacteria
260			_cagos. 2011
270			_a03/04/2012 ; 03/04/2012
300			_a6 p. ; 233 - 238
520			_aTranscripción del resumen del autor. A polymer-producing strain of Enterobacter sakazakii, designated JD, can excrete highly viscous water-insoluble polysaccharide at temperatures lower than 30 °C. Another strain, Bacillus subtilis I, can survive in harsh environments. These two strains were genetically recombined by protoplast fusion, and two excellent fusants designated STP-1 and STP-5 were obtained. The performances of biogas and extracellular polysaccharide production, as well as enhanced oil recovery, were evaluated by sand-pack flooding tests. STP-1 exhibited lower ability to produce biogas but higher capability of biopolymer production and enhanced oil recovery than STP-5. The decreasing percentage of the core permeability after microbe flooding was 83% by STP-1 and 58% by STP-5, respectively. STP-1 enhanced oil recovery by 25%, and STP-5 17%, from the saturated sand pack column. To the authors' knowledge, this is the first report about the construction of engineering bacteria which have the advantages of both high temperature resistance and high polysaccharide production.
581			_a2
773	0		_tJournal of Petroleum Science & Engineering _g78
942			_cARTICULO
999			_c186975 _d186975