000			01699nab a2200181 4500
005			20260520000837.0
008			260224s2009 xxu
245	0	0	_aBiomimetic CO2 sequestration: _b1. Immobilization of carbonic anhydrase within polyurethane foam
260			_a _b _cnov./dic. 2009
270			_a08/06/2010 ; 08/06/2010
300			_a5 p. ; 5725-5730
520			_aTranscripción del resumen del autor. Bovine carbonic anhydrase (CA) was immobilized within polyurethane (PU) foam for biomimetic CO2 sequestration. The catalytic activities for the free and immobilized CA were estimated using para-nitrophenyl acetate (p-NPA) as the substrate. Because the p-NPA has limited solubility in the aqueous phase, the activities were estimated in Tris buffer containing 10% acetonitrile. A Lineweaver-Burk relationship was employed to estimate the Michaelis-Menten kinetic parameters for the free and immobilized CA. The kcat, Km, and kcat/Km values for the free CA were found to be 2.02 s-1, 12.2 mM, and 166.4 M-1 s-1, respectively. The Km value for the immobilized CA was estimated to be 9.6 mM at the same conditions. The immobilized CA was stable and did not lose any activity over seven consecutive washings and activity tests. While the free CA lost its activity in 45 days stored at 4 °C in refrigerator, the immobilized CA maintained 100% of its activity over a 45 day period stored in Tris buffer at ambient conditions. It was concluded that the immobilized CA as a very stable biocatalyst could be employed in biomimetic CO2 sequestration.
581			_a6
773	0		_tEnergy & fuels _g23
942			_cARTICULO
100	1		_aOzdemir, Ekrem _942330
999			_c170809 _d170809