01674nab a2200193 4500 20260520002031.0 260224s2011 xxu Xu, Yaohui 52309 Lu, Mang 52310 Microbially enhanced oil recovery at simulated reservoir conditions by use of engineered bacteria agos. 2011 03/04/2012 ; 03/04/2012 6 p. ; 233 - 238 Transcripción del resumen del autor. A polymer-producing strain of Enterobacter sakazakii, designated JD, can excrete highly viscous water-insoluble polysaccharide at temperatures lower than 30 °C. Another strain, Bacillus subtilis I, can survive in harsh environments. These two strains were genetically recombined by protoplast fusion, and two excellent fusants designated STP-1 and STP-5 were obtained. The performances of biogas and extracellular polysaccharide production, as well as enhanced oil recovery, were evaluated by sand-pack flooding tests. STP-1 exhibited lower ability to produce biogas but higher capability of biopolymer production and enhanced oil recovery than STP-5. The decreasing percentage of the core permeability after microbe flooding was 83% by STP-1 and 58% by STP-5, respectively. STP-1 enhanced oil recovery by 25%, and STP-5 17%, from the saturated sand pack column. To the authors' knowledge, this is the first report about the construction of engineering bacteria which have the advantages of both high temperature resistance and high polysaccharide production. 2 Journal of Petroleum Science & Engineering 78 ARTICULO 186975 186975 0 0 0 0 254794 BAAB BAAB 2026-03-06 200059080 0 200059080 2026-03-06 00:15:37 2026-03-06 ARTICULO