01836nab a2200205 4500 20260520000928.0 260224s2011 xxu Biomimetic sequestration of CO2 and reformation to CaCO3 using bovine carbonic anhydrase immobilized on SBA-15 ene./feb. 2011 04/04/2011 ; 04/04/2011 8 p. ; 438–445 Transcripción del resumen del autor. The biocatalytic capture of CO2, and its precipitation as CaCO3, over bovine carbonic anhydrase (BCA) immobilized on a pore-expanded SBA-15 support was investigated. SBA-15 was synthesized using TMB as a pore expander, and the resulting porous silica was characterized by XRD, BET, IR, and FE-SEM analysis. BCA was immobilized on SBA-15 through various approaches, including covalent attachment (BCA-CA), adsorption (BCA-ADS), and cross-linked enzyme aggregation (BCA-CLEA). The effects of pH, temperature, storage stability, and reusability on the biocatalytic performance of BCA were characterized by examining para-nitrophenyl acetate (p-NPA) hydrolysis. The Kcat/Km values for p-NPA hydrolysis were 740.05, 660.62, and 680.11 M-1 s-1, respectively, whereas the Kcat/Km value for free BCA was 873.76 M-1 s-1. The amount of CaCO3 precipitate was measured quantitatively using an ion-selective electrode and was found to be 12.41, 11.82, or 11.28 mg CaCO3/mg for BCA-CLEA, BCA-ADS, or BCA-CA, respectively. The present results indicate that the immobilized BCA-CLEA, BCA-ADS, and BCA-CA are green materials, and are tunable, reusable, and promising biocatalysts for CO2 sequestration. 1 Energy & fuels 25 ARTICULO Vinoba, Mari 45086 Kim, Dae Hoon 45087 Lim, Kyoung Soo 45088 174421 174421 0 0 0 0 242240 BAAB BAAB 2026-03-05 200052437 0 200052437 2026-03-05 16:11:50 2026-03-05 ARTICULO